Viruses can only grow in the living cells. In order to study them, they need to be cultured in the living system. Chicken embryo techniques is used as the most economical and convenient for this purpose. In this technique, fertile chicken eggs incubated for 5 to 12 days are inoculated with the virus particles through the shell, aseptically. The opening may be sealed with paraffin wax. The eggs incubated at 36oC are ideal sources for the growth of viruses. Chick embryos contain several different types of cells in which various viruses will undergo replication. The yolk sac is a general ideal medium for the growth of viruses.