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Question

Choose the correct option regarding activity of different nucleases.



A
E1 - Endonuclease; E2 - Exonuclease; E3 - Restriction Endonuclease
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B
E1 - Exonuclease; E2 - Endonuclease; E3 - Restriction Endonuclease
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C
E1 - Exonuclease; E2 - Restriction Endonuclease; E3 - Endonuclease
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D
E1 - Restriction Endonuclease ; E2 - Exonuclease; E3 - Endonuclease
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Solution

The correct option is B E1 - Exonuclease; E2 - Endonuclease; E3 - Restriction Endonuclease
Exonucleases remove nucleotides from the ends of the DNA. They are used to create sticky ends or remove overhangs from a DNA molecule.

Endonucleases make cut at any position within the DNA.

Restriction endonucleases recognise a specific pallindromic nucleotide sequences and cut the both strands of DNA.

So, the correct option is:
E1 - Exonuclease; E2 - Endonuclease; E3 - Restriction Endonuclease

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