Question

Choose the correct option regarding activity of different nucleases.

• A. E1 - Endonuclease; E2 - Exonuclease; E3 - Restriction Endonuclease
• B. E1 - Exonuclease; E2 - Endonuclease; E3 - Restriction Endonuclease
• C. E1 - Exonuclease; E2 - Restriction Endonuclease; E3 - Endonuclease
• D. E1 - Restriction Endonuclease ; E2 - Exonuclease; E3 - Endonuclease

Answer 1

The correct option is B E1 - Exonuclease; E2 - Endonuclease; E3 - Restriction Endonuclease

Exonucleases remove nucleotides from the ends of the DNA. They are used to create sticky ends or remove overhangs from a DNA molecule.

Endonucleases make cut at any position within the DNA.

Restriction endonucleases recognise a specific pallindromic nucleotide sequences and cut the both strands of DNA.

So, the correct option is:
E1 - Exonuclease; E2 - Endonuclease; E3 - Restriction Endonuclease