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Question

Choose the correct statement(s) for light reaction:
I. Oxygen released during photosynthesis comes from molecules of carbon dioxide.
II. NADPH is a product of light reaction.
III. Photo-oxidation of water forms H+, O2 and e- .
IV. One photosynthetic unit consists of a photochemical reaction centre and antennae complex

A
I, III, IV
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B
II, III, IV
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C
Only II
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D
Only I and II
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Solution

The correct option is B II, III, IV
Oxygen released during photosynthesis comes from splitting of water molecules. It was proved initially with the help of indirect approach and later, by a direct evidence.

Indirect approach:
Photosynthesis in anoxygenic bacteria was compared with the photosynthesis in oxygenic bacteria.
nCO2 + nH2OChlorophyll & light−−−−−−−−−− (CH2O)n + nO2(Oxygenic organisms)
nCO2 + nH2SChlorophyll & light−−−−−−−−−− (CH2O)n + nS(Anoxygenic photosynthesis)

Direct evidence:
Two isotopes of oxygen - O16 and O18 were used.
nCO218 + nH2O16Chlorophyll & light−−−−−−−−−−−− (CH2O18)n + nO216

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