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Question

Choose the incorrect statement with reference to restriction enzymes.

A
The specific palindromic sequence which is recognized by EcoRI is
5′–CTTAAG–3′
3′–GAATTC–5′
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B
Restriction enzymes may cut the strands of DNA a little away from the centre of the palindrome sites
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C
Restriction endonucleases are used in genetic engineering to form ‘recombinant’ molecule of DNA
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D
These enzymes cut the strands of DNA between the same two bases on the opposite strands
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Solution

The correct option is A The specific palindromic sequence which is recognized by EcoRI is
5′–CTTAAG–3′
3′–GAATTC–5′
The specific palindromic sequence which is recognized by EcoRI is
5′ – GAATTC – 3′
3′ – CTTAAG – 5′

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