Question

Choose the vector which is used in gene mapping.

• A. Plasmid vector
• B. Phage vector
• C. Cosmid vector
• D. Yeast artificial chromosome (YAC)

Answer 1

The correct option is D Yeast artificial chromosome (YAC)

Yeast artificial chromosome (YAC) is used in gene mapping because larger size of foreign DNA (around 1 Mb) can be inserted in YAC.

All the other options have a smaller insert size and hence cannot be used. Plasmids can hold upto 7 kb, the insert size of a phage vector is upto 11 kb and for a cosmid vector it is upto 45 kb.