Question

Choose the wrongly matched pair from the following:

• A. Electrophoresis - an electrical pulse is used to create temporary pores in cell membranes
• B. Microinjection - rDNA is directly injected into the host cell
• C. Biolistics - cells are bombarded with high velocity micro-particles coated with DNA
• D. All are correct

Answer 1

The correct option is A Electrophoresis - an electrical pulse is used to create temporary pores in cell membranes

Gel electrophoresis is a technique used to separate macromolecules like DNA based on their size and charge in gel matrix under the influence of electric field.

Electroporation, biolistics and microinjection are methods of gene delivery into host cells directly. These are commonly classified under methods of transformation (the process of transfer of exogenous DNA into host cells).

• Electroporation - It uses electrical fields to destabilise the membrane for some time. This allows the entry of normally impermeable DNA into the cytoplasm.
• Biolistics/ gene gun - Plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA. Gold or tungsten is used as they are inert/ non-reactive in nature.
• Microinjection - Recombinant DNA is directly injected into the nucleus of an animal cell in this method.