The correct option is C Both B and C
Chromosome puffs : A swelling at a site along the length of a polytene chromosome; the site of active transcription. A diffuse uncoiled region in a polytene chromosome where transcription is actively taking place.
Puffs are characteristic of polytene chromosomes found in salivary glands of Drosophila and contain large quantities of mRNA. The appearance of puffs is correlated with the intense secretory activity which the larval salivary glands have to perform.