Cofactor (prosthetic group) is a part of holoenzyme. It is

Loosely attached inorganic part.

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Accessory non-protein substance attached firmly.

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Loosely attached organic part.

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None of the above.

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Solution

The correct option is C Accessory non-protein substance attached firmly.
Conjugate enzymes (eg dehydrogenase enzymes) have an additional non protein cofactor attached to the protein part. The cofactor can be organic or inorganic. Loosely attached organic cofactor are called coenzymes example NAD, FAD etc. while firmly attached one is prosthetic group example heme, biotin etc. The protein part of conjugate enzyme is known as apoenzyme while the whole enzyme is called as holoenzyme.

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