The correct option is B Acetocarmine
Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the onion root tip cells can be visualized perfectly by treating with this stain. Acetocarmine is a saturated solution of carmine in 45% acetic acid used especially for the rapid staining of fresh unfixed chromosomes. It is used to study the stages of cell division.
So, the correct option is C.