Question

Commonly used vectors for human genome sequencing are

• A. T/A Cloning Vectors
  
• B. T-DNA
  
• C. Expression Vectors
  
• D. BAC and YAC
  

Answer 1

The correct option is D BAC and YAC


Cloning Vectors
A cloning vector is a small piece of DNA, into which the foreign DNA or genetic material is inserted in recombinant DNA technology.

T/A cloning vectors
T/A cloning vectors are linearised plasmids which contain T overhangs at 3’ ends. These vectors are called T/A cloning vectors.
Any insert or gene containing A residue at the 5' end can be inserted in T vectors.

T-DNA
T-DNA is the region of the Ti-plasmid of Agrobacterium tumefaciens which contains genes for auxin, cytokinin, and opine synthesis.
T-DNA can be replaced with the foreign gene to introduce a foreign gene into the plant's genome.

BAC and YAC
BAC stands for Bacterial Artificial Chromosome and YAC stands for Yeast Artificial Chromosome.
● These are vectors used for cloning of genomic libraries because of their capacity to incorporate large insert sizes.
● These vectors are commonly used for genome sequencing.
Part 5: Expression vectors Expression vectors are plasmids which contain promoter sequences for the expression of genes of interest.

Final answer
(C) BAC and YAC