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Question

Compare the role of enzymes dna polymerase and dna ligase in replication fork of dna

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Solution

Dear Student

There are three DNA polymerase used in DNA replication.

DNA Polymerase 1 - repair polymerase involved in excision repair with 3' - 5' and 5' - 3' exonuclease activity. Present in prokaryotes.

DNA Polymerase II - 3' - 5' exonuclease activity, involved in DNA repair, replication restart to bypass lesions

DNA Polymerase III - holoenzyme, primary enzyme involved in DNA replication in E,Coli.

DNA ligase plays role in joining the Okazaki fragments of the lagging strand by providing the phosphodiester bond.

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