Question

Consider the following steps.
a. Isolation of tissue from the plant
b. Digestion of cell walls with cellular enzyme
c. Preparation of culture medium
d. Separation of the naked protoplasts culturing them in the medium
The correct sequence of the steps to be followed to culture protoplasts is?

• A. $1,2,3,4$
• B. $1,3,2,4$
• C. $4,1,2,3$
• D. $4,2,1,3$

Answer 1

Answer: (A)

$1,2,3,4$

A. 1,2,3,4

Solution : Culturing protoplasts is achieved first by isolating the tissue of interest from the plant and digestion of the cell wall with the help of cellulases.Then the appropriate culture medium for the growth of the cells is prepared followed by separation of the protoplasts and culturing them in the medium.

So the correct answer is "1,2,3,4"