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Question

Cry gene which synthesize crystal protein is isolated from _____________.

A
Bacillus thuringiensis
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B
Rhizobium
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C
Bacillus polymyxa
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D
Clostridium
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Solution

The correct option is A Bacillus thuringiensis
Bacillus thuringiensis (Bt) is a Gram-positive bacterium that produces crystals during sporulation. These crystals comprise ~130 kDa protoxins called crystal proteins which are potent and highly specific insecticides. Once ingested by specific host, these crystals dissolve in the alkaline conditions of the gut thereby activating the protoxins. The active toxins then bind to receptors on midgut epithelial cells, become inserted into the plasma membrane where they form pores leading to cell death through osmotic lysis. Option A is the correct answer.

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