The correct option is D Preservation at very low temperature
Cryopreservation is a process, where cells or any other substances susceptible to damage are preserved by cooling to sub-zero temperatures without causing additional damage caused by the formation of ice during freezing. At low temperature, all the enzymatic activities get stopped and decomposition of cells or tissues by inhibiting the growth of microbes at such temperature. Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants. Thus, the correct answer is option C.