1
Question
Cyanogen bromide is employed in
Cyanogen bromide is employed in
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Solution
The correct option is D Synthesis of humulin
Role of cyanogen bromide
Cyanogen bromide is an inorganic compound which is used during the production of recombinant insulin, humulin.
Humulin production involved the separate insertion of the chemically synthesised genes encoding human A and B peptides of insulin into a plasmid vector for expression in the host bacterium, E. coli. These genes are inserted adjacent to the gene encoding β-galactosidase in the plasmid. Separate batches of E. coli cells produced proinsulin chains, i.e., fused β-galactosidase-A chain and β-galactosidase-B-chain. These chains were separated from the stretch of β-galactosidase by the treatment of cyanogen bromide (CNBr).
Addition of an extra codon for methionine at N-terminal of each gene for the A and B chain helped in the action of CNBr and separation.
The A and B chains after joining in vitro are sulphonated to derive the mature functional insulin.
Genetic fingerprinting
Genetic fingerprinting is the process of determining nucleotide sequences of certain DNA areas which are unique to each individual. This can be used to distinguish between individuals.
Tissue culture
Tissue culture is the culturing of cells, tissues or organs of a plant or animal in a culture medium.
Plant tissue culture can be used to develop thousands of plants from a single plant cell through micropropagation.
Hybridoma technology
Hybridoma technology is the technology used in the production of monoclonal antibodies. The cell lines are formed by fusion of B cells that produce antibodies with myeloma cells.
Final answer:
(c) Synthesis of humulin
Role of cyanogen bromide
Cyanogen bromide is an inorganic compound which is used during the production of recombinant insulin, humulin.
Humulin production involved the separate insertion of the chemically synthesised genes encoding human A and B peptides of insulin into a plasmid vector for expression in the host bacterium, E. coli. These genes are inserted adjacent to the gene encoding β-galactosidase in the plasmid. Separate batches of E. coli cells produced proinsulin chains, i.e., fused β-galactosidase-A chain and β-galactosidase-B-chain. These chains were separated from the stretch of β-galactosidase by the treatment of cyanogen bromide (CNBr).
Addition of an extra codon for methionine at N-terminal of each gene for the A and B chain helped in the action of CNBr and separation.
The A and B chains after joining in vitro are sulphonated to derive the mature functional insulin.
Genetic fingerprinting
Genetic fingerprinting is the process of determining nucleotide sequences of certain DNA areas which are unique to each individual. This can be used to distinguish between individuals.
Tissue culture
Tissue culture is the culturing of cells, tissues or organs of a plant or animal in a culture medium.
Plant tissue culture can be used to develop thousands of plants from a single plant cell through micropropagation.
Hybridoma technology
Hybridoma technology is the technology used in the production of monoclonal antibodies. The cell lines are formed by fusion of B cells that produce antibodies with myeloma cells.
Final answer:
(c) Synthesis of humulin
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