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Question

Define and design a test cross.

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Solution

  • The crossing of an F1 progeny with a double (homozygous) recessive parental progeny is done in order to determine whether the progeny is homozygous or heterozygous for a character under consideration. This is called a test cross. It was first introduced by Gregor Mendel.
  • Those that are heterozygous will have one dominant and one recessive allele individuals and those that are homozygous dominant will have two dominant alleles, and those that are homozygous recessive will have two recessive alleles. A test cross is performed to determine the genotype of a dominant parent if it is a heterozygous or homozygous dominant.
  • On the basis of the results obtained in the ratio of the offspring, it can be predicted that the parent is having which genotype. If all the offsprings are of same phenotype, then the parent is homozygous dominant. If there is 1:1 ration in the phenotype of the organisms, the parent is heterozygous dominant.

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