Question

Define chromatography. Explain the underlying principle involved .

Answer 1

It is defined as the process of separation of the individual components of a mixture based on their relative affinities towards stationary and mobile phases
Principle:

The samples are subjected to flow by mobile liquid onto or through the stable stationary phase. The sample components are separated into fractions based on their relative affinity towards the two phases during their travel.

The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer.

THE UNDERLYING PRINCIPLE OF CHROMOTOGRAPHY

IS “SELECTIVE ADSORBTION”.