Define chromatography? Give examples
Dear Student
Chromatography is a technique used to separate mixtures into its components. In this method, a drop of mixture to be separated is placed on a filter paper, and the paper is hanged in a jar, slightly dipping in the solvent, so that when solvent rises up in the paper (as the filter papers has pores in it which allow the movement of the solvent), it carries the components of the mixture along with it. Different substances move at different speeds which allows the mixture to separate in its components.
This technique was first used for separation of colours, so this name was given
Practical application of chromatography is as follows:
- Used by doctors
- Scientists for analysis
- Analysis of polluted air.
The basic principle involved in chromatography is explained as:
The components to be separated are distributed between two phases: a stationary phase and a mobile phase which percolates through the stationary phase.
There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography.
Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers. It is used to analyze metal ions and organic compounds in solutions. Liquid chromatography uses liquids which may incorporate hydrophilic, insoluble molecules.
Gas Chromatography is used in airports to detect bombs and is used is forensics in many different ways. It is used to analyze fibers on a persons body and also analyze blood found at a crime scene. In gas chromatography helium is used to move a gaseous mixture through a column of absorbent material.
Thin-layer Chromatography uses an absorbent material on flat glass or plastic plates. This is a simple and rapid method to check the purity of an organic compound. It is used to detect pesticide or insecticide residues in food. Thin-layer chromatography is also used in forensics to analyze the dye composition of fibers.
Paper Chromatography is one of the most common types of chromatography. It uses a strip of paper as the stationary phase. Capillary action is used to pull the solvents up through the paper and separate the solutes
Regards
Dear Student
Chromatography is a technique used to separate mixtures into its components. In this method, a drop of mixture to be separated is placed on a filter paper, and the paper is hanged in a jar, slightly dipping in the solvent, so that when solvent rises up in the paper (as the filter papers has pores in it which allow the movement of the solvent), it carries the components of the mixture along with it. Different substances move at different speeds which allows the mixture to separate in its components.
This technique was first used for separation of colours, so this name was given
Practical application of chromatography is as follows:
- Used by doctors
- Scientists for analysis
- Analysis of polluted air.
The basic principle involved in chromatography is explained as:
The components to be separated are distributed between two phases: a stationary phase and a mobile phase which percolates through the stationary phase.
There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography.
Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers. It is used to analyze metal ions and organic compounds in solutions. Liquid chromatography uses liquids which may incorporate hydrophilic, insoluble molecules.
Gas Chromatography is used in airports to detect bombs and is used is forensics in many different ways. It is used to analyze fibers on a persons body and also analyze blood found at a crime scene. In gas chromatography helium is used to move a gaseous mixture through a column of absorbent material.
Thin-layer Chromatography uses an absorbent material on flat glass or plastic plates. This is a simple and rapid method to check the purity of an organic compound. It is used to detect pesticide or insecticide residues in food. Thin-layer chromatography is also used in forensics to analyze the dye composition of fibers.
Paper Chromatography is one of the most common types of chromatography. It uses a strip of paper as the stationary phase. Capillary action is used to pull the solvents up through the paper and separate the solutes
Regards
