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Question

Degenerated DNA after heating can be studied by comparing

A
A : T ratio
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B
G : C ratio
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C
Sugar : phosphate
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D
Number of nucleotides
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Solution

The correct option is D Number of nucleotides
Heating causes unwinding of dsDNA into a ssDNA molecule by altering the ordered stacking of the bases and breaking their connecting hydrogen bonds. Single stranded DNA do not obey the Chargaff's rule and thus, A : T and G : C ratio can not be taken into consideration. This ssDNA is then studied by comparing the type and number of nucleotides in single strands. Hence, correct option is D.

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