Some fine oil droplets were allowed to be sprayed into the chamber by an atomizer.
The air in the chamber was subjected to ionization by X rays. Electrons produced by the ionization of air attracted themselves to the oil drop.
When a sufficient amount of electric field is applied which can balance the gravitational force acting on an oil drop, the drop remains suspended in the air.
Millikan observed that the smallest charge found was approximately Coulomb and the charge on each drop was always an integral multiple of that value.
On the basis of this observation, he concluded that Coulomb is the smallest possible charge and consider that value as the charge of the electron.