Diagrammatically represent the experimental steps in cloning and expressing a human gene (say the gene for growth hormone) into a bacterium like E.coli?

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Diagram showing steps involved in cloning and expressing a hormone gene for growth hormone into E.coli.

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Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli ?

Q. Diagrammatically represent the experimental steps in cloning and expressing a human gene (say the gene for growth hormone) in a bacteria like E. coli?

Some of the steps involved in Gene Cloning are given below. Arrange them in correct order

(a) insertion of isolated gene to the vector (b) introduction of recombinant vector to the host

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Select the correct sequence of cloning method.

Q. The figure above illustrates gene expression in prokaryotic and eukaryotic cells:
Human insulin, a protein hormone that controls blood sugar levels, and many other eukaryotic proteins can be mass-produced by inserting eukaryotic genes into bacteria.
If an unaltered gene for human insulin was inserted into the DNA of a bacteria cell, how would the expression of the insulin gene be affected?

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