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Gene Cloning

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Question

Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli ?

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Solution

DNA cloning is a method of producing multiple identical copies of specific template DNA. It involves the use of a vector to carry the specific foreign DNA fragment into the host cell. The mechanism of cloning and transfer of gene for growth hormone into E.coli is represented below.

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Q. Diagrammatically represent the experimental steps in cloning and expressing a human gene (say the gene for growth hormone) into a bacterium like E.coli?

Q. Diagrammatically represent the experimental steps in cloning and expressing a human gene (say the gene for growth hormone) in a bacteria like E. coli?

Q. Select the options which will result in expression of a human gene into functional proteins.
I. cDNA for the protein is inserted into the genome of an E. coli host.
II. mRNA is inserted into the genome of an E. coli host.
III. Gene is inserted into the genome of Saccharomyces.
IV. Gene is inserted into the genome of E. coli host.

Q. Some of the steps involved in the production of human insulin are given below. Choose the correct sequence
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli

Q. pBR322, which is frequently used as a vector for cloning gene in E. coli is a/an

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