Differentiate between absorption spectrum and action spectrum.

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Solution

Absorption Spectrum Action Spectrum
The absorption spectrum indicates the wavelengths of light absorbed by each pigment (e.g. chlorophyll) The action spectrum indicates the overall rate of photosynthesis at each wavelength of light
It is a graphical representation of the amount of light of different wavelengths absorbed by a pigment. It is a graphical representation of the rate of photosynthesis at different wavelengths of light.
It is studied directly. It is studied in relation to either $O_{2}$ evolved or $C O_{2}$ absorbed.
It requires a single exposure to full light. It requires study of light utilization at various wavelengths.

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Action spectrum and absorption spectrum

Q. Is there a complete one-to-one overlap between the absorption spectrum of chlorophyll a and the action spectrum of photosynthesis?

Question 53
In the figure given below, the black line (upper) indicates action spectrum for photosynthesis and the lighter line (lower) indicates the absorption spectrum of chlorophyll-a, answer the following

(a) What does the action spectrum indicate? How can we plot an action spectrum? Explain with an example.

(b) How can we derive an absorption spectrum for any substance?

(c) If chlorophyll-a is responsible for light reaction of photosynthesis, why do the action spectrum and absorption spectrum not overlap?

Q. Can you say that there is a complete one - to - one overlap between the absorption spectrum of chlorophyll a and the action spectrum of photosynthesis? Justify your answer.

Q. What is emission spectrum and absorption spectrum? And what are the difference between them?

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Absorption Spectrum	Action Spectrum
The absorption spectrum indicates the wavelengths of light absorbed by each pigment (e.g. chlorophyll)	The action spectrum indicates the overall rate of photosynthesis at each wavelength of light
It is a graphical representation of the amount of light of different wavelengths absorbed by a pigment.	It is a graphical representation of the rate of photosynthesis at different wavelengths of light.
It is studied directly.	It is studied in relation to either $O_{2}$ evolved or $C O_{2}$ absorbed.
It requires a single exposure to full light.	It requires study of light utilization at various wavelengths.