Criteria | Prosthetic group | Co-enzyme |
Type of molecule | Either metal ions or small organic molecules. | Small organic molecules. |
Binding | Tightly-bound or stably-associated with the enzyme. | Loosely-bound to the enzyme. |
Role | Assists the functioning of the enzyme by binding with the apoenzyme. | Facilitates the biological transformation of the enzyme. |
Examples | Metal ions such as Co, Mg, Cu, Fe and organic molecules such as biotin and FAD. | Coenzyme A, biotin, folic acid, vitamin B12. |