Differentiation of callus requires a specific ratio of two hormones
A
Auxin and gibberellin
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B
Auxin and abscisic acid
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C
Gibberellin and abscisic acid
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D
IAA and cytokinin
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Solution
The correct option is D IAA and cytokinin Callus is an undifferentiated tissue in plants. According to Skoog and Miller, the differentiation of callus is influenced by the ratio of the growth hormones auxin and cytokinin. A high ratio of auxin (IAA) to cytokinin leads to initiation of roots whereas a low ratio leads to development of shoots. Auxins like IAA, NAA and IBA stimulate regeneration of roots and cytokinins like BAP (benzylaminopurine) and kinetin stimulate regeneration of shoots.