Discuss
with your teacher and find out how to distinguish between
(a) Plasmid
DNA and chromosomal DNA
(b) RNA
and DNA
(c) Exonuclease
and Endonuclease
Discuss with your teacher and find out how to distinguish between
(a) Plasmid DNA and chromosomal DNA
(b) RNA and DNA
(c) Exonuclease and Endonuclease
(a) Plasmid
DNA and chromosomal DNA
-
Plasmid DNA
Chromosomal DNA
Plasmid DNA is an
extra-chromosomal DNA molecule in bacteria that is capable of
replicating, independent of chromosomal DNA.
Chromosomal DNA is the entire DNA
of an organism present inside chromosomes.
(b) RNA
and DNA
-
RNA
DNA
1.
RNA is a single stranded
molecule.
DNA is a double stranded
molecule.
2.
It contains ribose sugar.
It contains deoxyribose sugar.
3.
The pyrimidines in RNA are
adenine and uracil.
The pyrimidines in DNA are
adenine and thymine.
4
RNA cannot replicate itself.
DNA molecules have the ability to
replicate.
5
It is a component of the
ribosomes.
It is a component of the
chromosomes.
(c) Exonuclease
and Endonuclease
-
Exonuclease
Endonuclease
It is a type of restriction
enzyme that removes the nucleotide from 5’ or 3’
ends of the DNA molecule.
It is a type of restriction
enzyme that makes a cut within the DNA at a specific site to
generate sticky ends.
(a) Plasmid DNA and chromosomal DNA
-
Plasmid DNA
Chromosomal DNA
Plasmid DNA is an extra-chromosomal DNA molecule in bacteria that is capable of replicating, independent of chromosomal DNA.
Chromosomal DNA is the entire DNA of an organism present inside chromosomes.
(b) RNA and DNA
-
RNA
DNA
1.
RNA is a single stranded molecule.
DNA is a double stranded molecule.
2.
It contains ribose sugar.
It contains deoxyribose sugar.
3.
The pyrimidines in RNA are adenine and uracil.
The pyrimidines in DNA are adenine and thymine.
4
RNA cannot replicate itself.
DNA molecules have the ability to replicate.
5
It is a component of the ribosomes.
It is a component of the chromosomes.
(c) Exonuclease and Endonuclease
-
Exonuclease
Endonuclease
It is a type of restriction enzyme that removes the nucleotide from 5’ or 3’ ends of the DNA molecule.
It is a type of restriction enzyme that makes a cut within the DNA at a specific site to generate sticky ends.
