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Restriction Enzyme

Distinguish b...

Question

Distinguish between Exonucleases and Endonucleases.

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Solution

Exonucleases are nuclease which cut off nucleotides from $5^{'}$ or $3^{'}$ ends of DNA molecule.
Endonucleases are nucleases which cleave DNA duplex at any point except the ends. Leading to blunt or sticky ends.

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Q. Discuss with your teacher and find out how to distinguish between.
(a) Plasmid DNA and Chromosomal DNA
(b) RNA and DNA
(c) Exonuclease and Endonuclease

Q. Mention the difference in the mode of action of exonuclease and endonuclease.

12. Discuss with your teacher and find out how to distinguish between:

(a) Plasmid DNA and Chromosomal DNA?

(b) RNA and DNA

(c) Exonuclease and endonuclease

Q. How is the action of exonuclease different from that of endonuclease?

Q. Assertion :Exonucleases remove nucleotides from the ends of DNA. Reason: Endonucleases make cuts as specific positions within the DNA.

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Restriction Enzyme

Standard XII Biology

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