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Question

DNA finger printing is related to

A
Techniques used in identification of finger prints of different persons
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B
Analysis of DNA samples using imprinting devices
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C
Techniques used for molecular analysis of different specimens of DNA
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D
None of the above
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Solution

The correct option is C Techniques used for molecular analysis of different specimens of DNA
DNA finger printing is a forensic technique which identifies the individuals by their DNA characteristics. The principle is that no two individuals share exact same nucleotide sequence, just like finger prints, hence the name DNA finger printing. The first step in DNA finger printing is the extraction of useable DNA followed by its digestion with restriction enzymes to produce the restriction fragments length polymorphisms or RFLPs which are then sorted according to size by gel electrophoresis. The sorted DNA fragments are then transferred from the gel to the surface of the nylon via southern blotting. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of the same composition thereby matching different DNA samples. The correct option is C.

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