6
Question
DNA finger printing technique was discovered by
DNA finger printing technique was discovered by
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Solution
The correct option is B A. Jeffreys
DNA finger printing is a forensic technique which identifies the individuals by their DNA characteristics. A variable number tandem repeat (or VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR are 10-100 bp long. This DNA polymorphism is revealed during DNA finger printing in creating DNA profile of individuals. It was invented by AIec Jeffreys. The first step in DNA finger printing is the extraction of usable DNA followed by its digestion with restriction enzymes to produce the restriction fragments length polymorphisms or RFLPs. The fragments are then sorted according to size by gel electrophoresis. The sorted DNA fragments are then transferred from the gel to the surface of the nylon via southern blotting. For the purpose, the agarose gel is mounted on filter paper wick and the hybridization membrane is sandwiched between the gel and any absorbent material, which draw the transfer buffer through the gel by capillary action. The buffer flow draws the DNA molecules which are then immobilized on the synthetic membrane. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of same composition. A photographic film on top of the nylon surface to make a genetic fingerprint.
DNA finger printing is a forensic technique which identifies the individuals by their DNA characteristics. A variable number tandem repeat (or VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR are 10-100 bp long. This DNA polymorphism is revealed during DNA finger printing in creating DNA profile of individuals. It was invented by AIec Jeffreys. The first step in DNA finger printing is the extraction of usable DNA followed by its digestion with restriction enzymes to produce the restriction fragments length polymorphisms or RFLPs. The fragments are then sorted according to size by gel electrophoresis. The sorted DNA fragments are then transferred from the gel to the surface of the nylon via southern blotting. For the purpose, the agarose gel is mounted on filter paper wick and the hybridization membrane is sandwiched between the gel and any absorbent material, which draw the transfer buffer through the gel by capillary action. The buffer flow draws the DNA molecules which are then immobilized on the synthetic membrane. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of same composition. A photographic film on top of the nylon surface to make a genetic fingerprint.
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