Question

DNA fragments result when.......... cut DNA molecules at specific sites.

• A. RFLPs
• B. DNA probes
• C. restriction enzymes
• D. DNA polymerase

Answer 1

The correct option is C restriction enzymes

• RFLP- Restriction fragment length polymorphism: RFLP is a difference in homologous DNA sequence that can be detected by the presence of fragments of different length after digestion of DNA sample by restriction enzymes. This technique is utilized in genetic fingerprinting and paternity testing.

• DNA probes: DNA probes are used to identify and label DNA fragments that contain a specific sequence. A probe is simply a short length of DNA (20-100 nucleotide length) with a label attached.
  
• Restriction enzyme: A restriction enzyme is an enzyme that belongs to a larger group of an enzyme called nucleases and recognizes a specific nucleotide sequence and cuts the DNA only at a specific site which is known as the restriction site.
  
• DNA polymerase: It is an enzyme that synthesizes DNA molecules from deoxyribonucleotides the building block of DNA.
  

So, the correct option is 'restriction enzymes'.