DNA fragments with sticky ends are not allowed to undergo self ligation by

Unwindase

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Single strand binding proteins

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Gyrase

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Alkaline phosphatase

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Solution

The correct option is D Alkaline phosphatase
The correct option is D.
DNA fragment with sticky end are not allowed to undergo self ligation by Alkaline phosphatase. Alkaline phosphatase is a useful enzyme in genetic engineering, it remove $5^{'}$ phosphase group from DNA and RNA fragments. It also removes the $5^{'}$ phosphase groups from nucleotide and proteins.

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