DNA histone complex can be removed from chromosome by treatment with
A
6MNaCI+Mg[OH]3
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B
1 M NaCl
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C
Mg[OH]3
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D
Non -Separable
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Solution
The correct option is B 1 M NaCl 0.1 M - 0.2 M NaCl is the solution of intermediate ionic strength. The solution can be used to separate the DNA and histone proteins from the chromatin network. 0.6 M NaCl removes H1 and higher concentration like 1 M NaCl helps in the removal of all the histone proteins and DNA. At further higher concentrations, the renaturation of DNA will be difficult and so higher concentrations cannot be used.