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Question

DNA is a polymer of nucleotides which are linked to each other by 3-5 phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?

A
Replace purines with pyrimidines.
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B
Remove/Replace 3 OH group in deoxyribose.
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C
Remove/Replace 2 OH group with some other group in deoxyribose.
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D
Both (b) and (c).
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Solution

The correct option is B Remove/Replace 3 OH group in deoxyribose.
If 3 OH group is removed/replaced in deoxyribose, there will be no formation of pliosphodiester bonds and hence polymerisation of nucleotides will be prevented.

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