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Question

DNA-mRNA hybrids are made using

A
Reverse transcriptase
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B
DNA polymerase
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C
RNA polymerase
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D
DNA hybridase
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Solution

The correct option is D Reverse transcriptase
Complementary DNA (cDNA) is DNA made from mRNA. This makes use of the enzyme reverse transcriptase, which does the reverse of transcription. It synthesises DNA from an RNA template. It is produced naturally by a group of viruses called retroviruses (which include HIV), and it helps them to invade cells. In genetic engineering, reverse transcriptase is used to make an artificial gene of cDNA.
Therefore, the correct answer is option A.

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