DNA polymerase adds nucleotides to the free ___ end of RNA primers.

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Solution

DNA polymerase can add nucleotides only to the free 3’ end of RNA primers. Thus it works exclusively in the 5’ $\to$ 3’ direction, which basically means that DNA replication follows the 5’ $\to$ 3’ direction.

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Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Match the following enzymes of DNA replication with their respective functions:

p. DNA polymerase i. Helps unzip the DNA double helix

q. DNA ligase ii. Seals nicks between Okazaki fragments

r. DNA helicase iii. Lays RNA primers to which polymerase can add nucleotides

s. Primase iv. Adds nucleotides to the free 3’ end of RNA primers

Q. DNA polymerase adds nucleotides to the 3’ end of a primer, extending the new chain in a 5’ → 3’ direction.

Q. Match the following enzymes of DNA replication with their respective functions:

\begin{matrix} Column I & Column II p. DNA polymerase & i. Helps unzip the DNA double helix q. DNA ligase & ii. Seals nicks between Okazaki fragments r. DNA helicase & iii. Lays RNA primers to which polymerase can add nucleotides s. Primase & iv. Adds nucleotides to the free 3' end of RNA primers \end{matrix}

Q. A biology student is performing PCR for the first time. She adds the right DNA fragments and its corresponding primers. She also adds DNA polymerase and free nucleotides. The reaction mixture is kept in a water bath that measures

37^{\circ} C

. But DNA amplification does not occur. Why?

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DNA polymerase adds nucleotides to the free ___ end of RNA primers.

DNA polymerase can add nucleotides only to the free 3’ end of RNA primers. Thus it works exclusively in the 5’→3’ direction, which basically means that DNA replication follows the 5’→3’ direction.

DNA polymerase can add nucleotides only to the free 3’ end of RNA primers. Thus it works exclusively in the 5’ $\to$ 3’ direction, which basically means that DNA replication follows the 5’ $\to$ 3’ direction.