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Standard XII
Biology
EtBr Usage
DNA strands o...
Question
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :
A
Bright blue bands
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B
Yellow bands
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C
Bright orange bands
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D
Dark red bands
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Solution
The correct option is
C
Bright orange bands
Ethidium bromide is a dye used in gel electrophoresis which is a technique used to separate DNA fragments.
Ethidium bromide intercalates between the base pairs of double stranded DNA and appear as bright orange coloured bands when exposed to UV light.
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Q.
Separated bands of DNA in agarose gel electrophoresis observed under UV radiation are stained with:
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DNA staining by ethidium bromide is followed by the exposure to UV rays. Now DNA is seen as bands of :
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A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
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Assertion :Gel electrophoresis and elution are two important processes. Reason: After staining with ethidium bromide, it has to be exposed to UV light.
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Ethidium bromide is a stain that binds specifically to nucleic acids (DNA or RNA). If viewed under ultraviolet light, it gives ________ color to the nucleic acid bands.
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