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Question

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as


A

Yellow bands

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B

Dark red bands

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C

Bright blue bands

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D

Orange bands

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Solution

The correct option is D

Orange bands


The correct option is option D.

Explanation for correct option:

  1. DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear bright orange.
  2. They appear orange because ethidium bromide releases an orange fluorescence at 605nm when exposed to UV light.
  3. Ethidium bromide is the intercalating agent that stacks in between the nitrogenous bases.

Final answer: DNA strands on a gel stained with ethidium bromide appear bright orange when viewed under UV radiation.


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