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PCR in Diagnosis

Does DNA poly...

Question

Does DNA polymerase need a primer?

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Solution

DNA polymerase:
The enzyme that duplicate genetic information stored in the DNA (Deoxyribonucleic acid) is called DNA polymerase.
It requires primer as they can only add nucleotides to the growing polypeptide chains.
It cannot initiate replication.

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Q. How many of the following statements are correct ?

A. DNA dependent DNA polymerase requires DNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

B. DNA dependent DNA polymerase requires RNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

C. During replication, unwinding of DNA double helix forms a fork-like structure called replication fork.

D. During transcription, unwinding of the DNA double helix by DNA dependent RNA polymerase forms a transcription bubble.

E. During transcription, unwinding of DNA double helix by DNA dependent RNA polymerase forms a transcription fork.

DNA polymerase adds nucleotides to the free ___ end of RNA primers.

Q. Does DNA replication in bacteria need a primer?

Mention the number of primers required in each cycle of polymerase chain reaction. Write the role of primers and DNA polymerase in PCR.

Q. (a) Mention the number of primers required in each cycle of polymerase chain
reaction (PCR). Write the role of primers and DNA polymerase in PCR., (b) Give the characteristic feature and source organism of the DNA polymerase in PCR.

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