Question

Downstream process: purification :: Upstream process:_______

• A. Biosynthesis
• B. Sedimentation
• C. Centrifugation
• D. Filtration

Answer 1

The correct option is A Biosynthesis

Since the upstream process is the first half of the process for obtaining recombinant proteins, this step can be considered a biosynthetic phase. In this process a suitable culture is isolated and enriched so that they can produce recombinant proteins in both good quantity and quality.
Once the product is produced in bioreactors, it is followed by downstream processes i.e filtration (separation of microbial cell from culture medium), centrifugation (separation of components in mixture on the basis of their densities) and sedimentation (keeping the culture media undisturbed for a long period. The heavier cellular debris settles at the bottom and culture medium with the recombinant protein becomes the supernatant.