The correct option is A Primary
Primary structure of a protein refers to the linear chain of amino acids joined together by peptide bonds. Disruption of the primary structure occurs by chemical cleavage of a linear chain of amino acids. Physical shock like heating, subjecting to high pressure and extremes of pH, etc, will denature the protein, i.e., distort secondary, tertiary or quarternary structure but cannot break the linear chain of amino acids because secondary, tertiary and quaternary structures are formed by the sulfide bond, hydrogen bond, Vanderwall's forces etc.