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Helicase

during dna re...

Question

during dna replication the strands are seperated by :
1.Unwindase
2.Topo isomerase
3.DNA polymerase
4.Gyrase
i wonder that the answer must be topo isomerase right.and also gyrase is a type of isomerase .

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Solution

DNA Helicase Also known as helix destabilizing enzyme. Helicase separates the two strands of DNA at the Replication Fork behind the topoisomerase. so the answer should be DNA helicase but since that is not in option so DNA polymerase is the correct answer.

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A. Helicase	(i) To join nucleotides on DNA
B. Gyrase	(ii) To open DNA
C. Primase	(iii) Unwind DNA
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Q. Match List I(Enzyme) with List II(Function) and select the correct answer using the codes given the lists.

List-I	List-II
a. DNA Ligase	$1$ . Catalyses the covalent union of segments of an interrupted sugarphosphate strand in double-stranded DNA
b. DNA polymerase	$2$ . End nuclease that digests either single stranded or double stranded DNA
c. DNase I	$3$ . Produces single or double stranded breaks in DNA during replication to release tension brought about by the separation of double strands
d. DNA topoisomerase	$4$ . Catalyses the formation of DNA worn deoxyribonucleotides using one strand of DNA as template

Q. Match List I with List II and select the correct answer.

List-I (Enzyme)	List-II( Conversions)
a. Phosphogluco isomerase	$1$ . Fructose $\to$ Glucose
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