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Question

During gene amplification the mixture of sample DNA, nucleotides and polymerases is heated to

A
Activate polymerases
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B
Activate nucleotides
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C
Separate sense and antisense DNA strands
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D
Provide energy for different strands
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Solution

The correct option is A Separate sense and antisense DNA strands
PCR is an enzymatic process in which a specific region of DNA is replicated over and over again to yield many copies of a particular sequence. This molecular “xeroxing” process involves heating and cooling samples in a precise thermal cycling pattern over ≈30 cycles which causes to separate sense and antisense DNA strands.
So, the correct answer is 'Separate sense and antisense DNA strands.'

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