1
Question
During Meselson and Stahl's experiments, heavy DNA was distinguished from normal DNA by which type of centrifugation?
During Meselson and Stahl's experiments, heavy DNA was distinguished from normal DNA by which type of centrifugation?
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Solution
The correct option is D CsCl gradient
In the following procedure, centrifugation is done on a CsCl gradient, the DNA separate out at a point when density equals that of the salt solution. 15NH4Cl was used as a heavy isotope of ammonium chloride while, 14NH4Cl was used as the light isotope of ammonium chloride & the type of centrifugation used was density gradient centrifugation in a CsCl medium. So, the correct answer is "CsCl gradient".
In the following procedure, centrifugation is done on a CsCl gradient, the DNA separate out at a point when density equals that of the salt solution.
15NH4Cl was used as a heavy isotope of ammonium chloride while, 14
NH4Cl was used as the light isotope of ammonium chloride & the type of centrifugation used was density gradient centrifugation in a CsCl medium. So, the correct answer is "CsCl gradient".
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