Question

During PCR technique, the pairing of primers to ssDNA segment is known as

• A. Denaturation
• B. Annealing
• C. Polymerisation
• D. Isolation

Answer 1

Answer: (B)

Annealing

• PCR - technique amplifies the DNA sample in three steps namely denaturation of target DNA, annealing and polymerization.
• Denaturation refers to the thermal cycle that separates the DNA strands and forms the ssDNA.
• Annealing refers to the pairing of primers to the ssDNA and polymerization is the extension of primer into complete DNA strand complementary to the template strand.

So, the correct answer is option B.