Question

Eco RI is

• A. terminal transferase
• B. restriction enzyme
• C. vector plasmid
• D. vector virus

Answer 1

The correct option is B restriction enzyme

Eco RI is first endonuclease enzyme which has been isolated from the Rly 13 strain of Escherichia coli bacterium. The cleavage site for EcoRI is as follows
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
The cleavage product is the follwoing in the form of sticky ends –
5’ – G AATTC – 3’
3’ – CTTAA G – 5’