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Question

Eco RI is

A
terminal transferase
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B
restriction enzyme
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C
vector plasmid
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D
vector virus
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Solution

The correct option is B restriction enzyme
Eco RI is first endonuclease enzyme which has been isolated from the Rly 13 strain of Escherichia coli bacterium. The cleavage site for EcoRI is as follows
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
The cleavage product is the follwoing in the form of sticky ends –
5’ – G AATTC – 3’
3’ – CTTAA G – 5’

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