Question

EDTA is commonly used in tissue culture. It is a

• A. Vitamin
• B. Hormone
• C. Nutrient
• D. Buffer

Answer 1

The correct option is D Buffer

EDTA stands for Ethylenediaminetetraacetic acid. It is widely used for both industrial and medical purposes. It is a buffer. Tris EDTA buffer is used in tissue culture as divalent cations chelator. It binds to calcium and thus prevents adherence of cells to each other. It also prevents aggregation of cells in cell suspension by binding to calcium and magnesium ions present in tissue culture media.

Thus, the correct answer is option D.