Question

Electroporation procedure involves

• A. Fast passage of food through sieve pores in phloem elements with the help of electric stimulation.
• B. Opening of stomatal pores during night by artificial light.
• C. Making transient pores in the cell membrane to introduce gene constructs.
• D. Purification of saline water with the help of a membrane system.

Answer 1

The correct option is C Making transient pores in the cell membrane to introduce gene constructs.

Electroporation, or electropermeabilization, is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane (electric pulses create transient pores in the cell membrane), allowing chemicals, drugs, or DNA to be introduced into the cell. In molecular biology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA. Electroporation is also highly efficient for the introduction of foreign genes into tissue culture cells.