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Question
ELISA is used to detect viruses, where
ELISA is used to detect viruses, where
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Solution
The correct option is D Alkaline phosphatase is the key reagent
The enzyme-linked immunosorbent assay (ELISA) is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. ELISA can be applied to the determination of serum antibody concentrations in a virus test. In this test antigen coated well is interact with antigen-specific antibody after incubation it is bind with the enzyme linked antibody and then colour containing substrate is added to detect the reaction by colour formation. In this method horse reddish peroxidase and alkaline phosphatase are used. Thus, the correct answer is option B.
The enzyme-linked immunosorbent assay (ELISA) is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. ELISA can be applied to the determination of serum antibody concentrations in a virus test. In this test antigen coated well is interact with antigen-specific antibody after incubation it is bind with the enzyme linked antibody and then colour containing substrate is added to detect the reaction by colour formation. In this method horse reddish peroxidase and alkaline phosphatase are used. Thus, the correct answer is option B.
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